Characteristics of Botrylloides violaceus

Botrylloides violaceus
Colony
Shape flat
Thickness (mm) 2.0-3.0
Arrangement of zooids ladder
Tunic transparent, gelatinous, soft,
Ampullae size (mm: L x W) 0.5 x 0.2
Formation of colony part including only vascular system infrequently
Color of pigment cells forming pattern on a colony surface no
Pattern by deposited pigment cells single pattern
Zooid
Length (mm) 2.5-3.0
Ground color brown , red, pale yellow, orange, or dark brown
Number of tentacles (Large),(Small),(Minute) L4, S4, M2-3
Number of stigmatal rows 10-11
Second row of stigmata incomplete
Number of longitudinal vessels 3
Number of stomach plication 9
Pyloric caecum small
Asexual reproduction
Peribranchial budding all seasons
Number of buds in each zooid R2, L2
Vascular budding infrequently
Cycle of takeover 5-6 days
Sexual reproduction
Breeding season May-Aug
Arrangement of gonads (position of ovary) posterior to the testis
Testis shape & color rosette, grayish
Color of eggs yellow
Production of larvae viviparous
Mature egg diameter (µm) <100
Number of mature eggs R1, L1
Brooding organ pouch formed from atrial wall
Embryo size just before hatching (µm) 1000-1200
Development to larvae more than one month
Release of larvae after 6-7 times of takeover of blastozooids
Larva
Length (mm) 3
Length of larval trunk (µm) 1000
Number of larval ampullae 24-34
Oozooid
Length (µm) -
Number of tentacles -
Number of protostigmata -
Number of stomach plication -
Number of buds R2, L1
Colony specificity
Present or absent present
Starting stage of allorejection before tunic fusion
Occurrence of subcuticular rejection yes
References Saito et al., 1981b
- : not examined

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