Characteristics of Botrylloides lenis

Botrylloides lenis
Colony
Shape flat
Thickness (mm) 1.7-2.0
Arrangement of zooids ladder
Tunic tranparent, gelatinous, very soft
Ampullae size (mm: L x W) 0.6 x 0.17
Formation of colony part including only vascular system sometimes
Color of pigment cells forming pattern on a colony surface dark brown
Pattern by deposited pigment cells single pattern
Zooid
Length (mm) 2.0-2.3
Ground color yellow, orange, brown, dark brown, or dark orange
Number of tentacles (Large),(Small),(Minute) L4, S4
Number of stigmatal rows R9, L8
Second row of stigmata incomplete
Number of longitudinal vessels 3
Number of stomach plication 8
Pyloric caecum small
Asexual reproduction
Peribranchial budding all seasons
Number of buds in each zooid R1, L1
Vascular budding sometimes
Cycle of takeover 6-7 days
Sexual reproduction
Breeding season Jul-Oct
Arrangement of gonads (position of ovary) posterior to the testis
Testis shape & color rosette, grayish
Color of eggs pinkish or orange
Production of larvae viviparous
Mature egg diameter (µm) 90-100
Number of mature eggs R1, L1
Brooding organ pouch formed from atrial wall
Embryo size just before hatching (µm) 750-800
Development to larvae in 20 days
Release of larvae 2 weeks after degeneration of parent zooids
Larva
Length (mm) 2.2-2.4
Length of larval trunk (µm) 700
Number of larval ampullae 14-24
Oozooid
Length (µm) -
Number of tentacles -
Number of protostigmata 8
Number of stomach plication -
Number of buds R1
Colony specificity
Present or absent present
Starting stage of allorejection before tunic fusion
Occurrence of subcuticular rejection -
References Saito & Watanabe, 1985
- : not examined

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