No.24-01
棘皮動物における細胞の多分化能性•再性能メカニズムの研究
代表研究者：BROWN UNIVERSITY Investigator 矢島麻美子
- Oulhen , N., Yoshida, T., Yajima, M., Song, J., Sakuma, T., Sakamoto, N., Yamamoto, T., Wessel, G.M. (2013) The 3'UTR of Nanos2 directs enrichment in the germ cell lineage of the sea urchin. Dev. Biol. In press.
- Yajima, M., Suglia, E., Gustafson, E.A. and Wessel, G.M. (2013) Meiotic gene expression initiates during larval development in the sea urchin. Dev. Dyn. 242(2):155-163.
- Yajima, M. and Wessel, G.M. (2012) Autonomy in Specification of Primordial Germ Cells and their Passive Translocation in the Sea Urchin. Development 139: 3786-3794.
- Yajima, M., Fairbrother, W. and Wessel, G.M. (2012) ISWI contributes to ArsI insulator function in development of the sea urchin. Development 139: 3613-3622.
No.24-03 ウニ卵レクチン(SUEL)ファミリーのグライコーム研究
代表研究者： 横浜市立大学大学院生命ナノシステム科学研究科 教授 大関泰裕
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Fujii Y., Sugawara S., Araki D., Kawano T., Tatsuta T., Takahashi K., Kawsar S M A., Matsumoto R., Kanaly RA., Yasumitsu H., Ozeki Y., Hosono M., Miyagi T., Hakomori S-I., Takayanagi Y., Nitta K. MRP1 expressed on Burkitt's lymphoma cells was depleted by catfish egg lectin through Gb3-glycosphinogolipid and enhanced cytotoxic effect of drugs. Protein J. 31, (1) 15-26. (2012)
- Fujii Y., Dohmae N., Takio K., Kawsar S. M. A., Matsumoto R., Hasan I., Koide Y., Kanaly R. A, Yasumitsu H., Ogawa Y., Sugawara S., Hosono M., Nitta K., Hamako J., Matsui T., Ozeki Y. A lectin from the mussel Mytilus galloprovincialis has a highly novel primary structure and induces glycan-mediated cytotoxicity of globotriaosylceramide-expressing lymphoma cells. J. Biol. Chem. 287, 44772-44783. (2012)
- Ozeki Y, Kawsar S. M. A., Fujii Y, Ogawa Y, Sugawara S, Hasan I, Koide Y, Yasumitsu H, Kanaly R A.:Chapter 8: Ability of diverse marine invertebrate lectins to regulate cell functions. "Marine Proteins and Peptides: Biological Activities and Applications" Se-Kwon Kim (ed), p167-184. Wiley-Blackwell Inc. ISBN: 978-1-1183-7506-8 (2013)
- Matsumoto R., Fujii Y., Kawsar S M A., Kanaly R A., Yasumitsu H., Koide Y., Hasan I., Ogawa Y., Im CH., Sugawara S., Hosono M., Nitta K., Hamako J., Matsui T., Ozeki Y. Cytotoxic and glycan-binding properties of an 18 kDa lectin isolated from the marine sponge Halicondria okadai. Toxins 4, 323-338. (2012)
- Yamamoto-Suzuki Y., Sakurai Y., Fujimura Y., Matsumoto M., Hamako J., Kokubo T., Kitagawa H., Kawsar S M A., Fujii Y., Ozeki Y., Matsushita F., Matsui T. Identification and recombinant analysis of btrocetin-2, a snake venom cofactor for von Willebrand factor-induced platelet agglutination. Biochemistry 51, 5329-5338 (2012)
- Yasumitsu H., Tajima H., Isobe M., Kutsna S., Kawsar S. M. A., Fujii Y., Kanaly R. A., Ozeki Y., Yokota E. Fine bubble mixing (FBM) culture of E. coli: a highly cost-effective middle scale-size culture system. Protein Pept. Lett. 20, 213-217. (2013)
No.24-07 海産無脊椎動物および脊椎動物のカルシトニンの構造及び生理的役割：特に円口類について
代表研究者： 金沢大学環日本海域環境研究センター臨海施設 准教授 鈴木信雄
- Suzuki, N., Sekiguchi, T., Satake, H., Kato, K., Nishiyama, Y., Takahashi, H., Danks, J.A., Martin, T.J., Hattori, A., Nakano, M., Kakikawa, M., Yamada, S., Ogoshi, M., Hyodo, S., Yamaguchi, Y., Chowdhury, V.S., Hayakawa, K., Funahashi, H., Sakamoto, T., and Sasayama, Y.: Cloning of two members of the calcitonin receptor family from stingray, Dasyatis akajei: Possible physiological roles of the calcitonin family in osmoregulation. Gene, 499: 90-95 (2012)
No.24-08 人工ヌクレアーゼを利用したカタユウレイボヤの遺伝子ノックアウト技術の開発
代表研究者： 広島大学大学院理学研究科 教授 山本 卓
- Hozumi A, Yoshida R, Horie T, Sakuma T, Yamamoto T and Sasakura Y. Enhancer activity sensitive to the orientation of the gene it regulates in the chordate genome. Dev Biol, in press (2013)
- Sakuma T, Hosoi S, Woltjen K, Suzuki KI, Kashiwagi K, Wada H, Ochiai H, Miyamoto T, Kawai N, Sasakura Y, Matsuura S, Okada Y, Kawahara A, Hayashi S and Yamamoto T. Efficient TALEN construction and evaluation methods for human cell and animal applications. Genes Cells, 18: 315-326, 2013
- Kawai N, Ochiai H, Sakuma T, Yamada L, Sawada H, Yamamoto Tand Sasakura Y．Efficient targeted mutagenesis of the chordate Ciona intestinalis genome with zinc-finger nucleases. Dev Growth Differ, 54: 535-545, 2012
No.24-11 ドジョウ二倍性精子の機能に関する研究
代表研究者： 北海道大学大学院水産科学研究院施設 教授 荒井克俊
- Zhao Y, Psenicka M, Fujimoto T, Saito T, Yasui GS, Yamaha E, Arai K.：Motility, morphology, mitochondria and ATP content of diploid spermatozoa from sex-reversed clonal diploid and neo-tetraploid loaches, Misgurnus anguillicaudatus. Journal of Applied Ichthyology, 28:1006-1012 (2012) 10.1111/jai.12069
- Zhao Y, Saito T, Pšenička M, Fujimoto T, Arai K. Comparison of spermatozoa parameters, fine structures, and energy-related factors among tetraploid, hyper-tetraploid, and hyper-triploid loaches (Misgurnus anguillicaudatus) J Exp Zool A Ecol Genet Physiol. 2014 Apr;321(4):198-206. doi: 10.1002/jez.1851. Epub 2014 Jan 30.
No.24-23 遺伝子改変マウスを用いた精子鞭毛運動調節機構に関する研究
代表研究者： 大阪大学微生物病研究所 教授 伊川正人
- Satouh Y, Inoue N, Ikawa M, Okabe M. (2012) Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1. J Cell Sci. [Epub ahead of print].
